Faculty & Research

François Baneyx

François Baneyx

 

François Baneyx

Department Chair and Charles W.H. Matthaei Professor of Chemical Engineering

Adjunct Professor of Bioengineering

 

Office: 107 Benson
Phone: 206-685-7659
Fax: 206-685-3451 or 206-543-3778
E-mail: baneyx@uw.edu
Lab website: Baneyx Group Website

 

Education

  • Ingénieur, E.N.S.I.G.C., Toulouse (France), 1987.
  • Ph.D., University of Texas (Austin), 1991.
  • Postdoctoral Fellow, Du Pont (Wilmington), 1991.

 

Research Interests

  • Protein folding, protein engineering, protein expression
  • Bionanotechnology, molecular biomimetics
Research in the Baneyx laboratory lies at the intersection of engineering, biology and nanotechnology. Current areas of focus include:

Protein-aided materials synthesis and assembly

Living organisms fabricate inorganic materials with outstanding mechanical, transport, optical, and magnetic properties. They do so under ambient conditions of temperature and pressure, using biomacromolecules (and especially proteins) to control nucleation, growth, shape, composition, and crystallinity, often in a hierarchical manner and over scales that range from nanometers to millimeters. We seek to emulate and surpass these feats of nature by understanding how combinatorially-selected solid binding peptides (SBPs) interact with inorganic substrates, and by genetically installing such SBPs within the framework of rationally or computationally designed proteins. We use the resulting chimera to control inorganic morphogenesis, endow inorganic nanostructures with biological attributes, and fabricate hybrid architectures with unique functions under sustainable and environmentally friendly conditions. Applications include biosensing and bioimaging, controlled drug delivery, vaccine technology, protein purification, and protein-guided assembly of post-silicon electronics.

Bionanoelectronics and bioinspired energy conversion

Energy transduction at the nanoscale is key for developing ever smaller self-powered biotic-abiotic circuitry. In biological systems, chemical, mechanical, and electromagnetic energy is converted from one form to another using ubiquitous proton gradients. For instance, bacteriorhodopsins harness light energy to pump protons outwards and generate a proton motive force (PMF) across cell membrane, while ATPases exploit inwards proton transport to synthesize ATP from ADP. In collaboration with the Rolandi lab at UCSC, we develop methodologies to directionally integrate such proteins within artificial membranes and onto proton-conducting contacts. We build devices in which proton gradients can be modulated with light and converted into electronic signals and seek to understand and exploit other aspects of bioinspired energy conversion. Applications include sensing, visual prostheses, bionanoelectronics and drug delivery.

Designed protein arrays

Biological building blocks that self-assemble into predetermined supramolecular structures hold promise for the production of advanced materials, devices and systems. However, our ability to predict, engineer and control short- and long-range interactions in proteins is lagging and nature’s most versatile building block remains underused. In collaboration with the Baker lab at UW, we use a family of computationally designed proteins to build 2- and 3D lattices that retain short-range order at the nanoscale but grow over tens to hundreds of micrometers. By controlling the adsorption of these arrays at technological-relevant surfaces through the use of SBPs, we explore their potential as tunable molds for inorganic mineralization and scaffolds for the high- density or controlled display of enzymes and proteins. Applications range from adoptive T cell therapy for cancer treatment to combining enzymatic and heterogeneous catalysis on a single platform.


Recent Publications

  • Z. Hemmatian, S. Keene, E. Josberger, T. Miyake, C. Arboleda, J. Soto-Rodriguez, F. Baneyx and M.  Rolandi. 2016. Electronic control of H+ current in a bioprotonic device with Gramicidin A and Alamethicin. Nat. Commun. 7:12981.

  • W. Yang, B. Hellner and  F. Baneyx. 2016. Self-immobilization of Car9 fusion proteins within high surface area silica sol-gels and dynamic control of protein release. Bioconjug. Chem. 27:2450-59.

  • Soto-Rodriguez, J., Z. Hemmatian, E. Josberger, M. Rolandi and F. Baneyx. 2016. A palladium-binding deltarhodopsin for light-activated conversion of protonic to electronic currents. Adv. Mater. 28:6581-6585.

  • Coyle, B.L. and F. Baneyx. 2016. Direct and reversible immobilization and microcontact printing of functional proteins using a genetically appended silica-binding tag. Chem. Commun. 52:7001-7004.

  • Liu, E.J., Sinclair, A., Keefe, A.J., Nannenga, B.L., Coyle, B.L., Baneyx, F. and S. Jiang. 2015. EKylation: Addition of an alternating-charge peptide stabilizes proteins. Biomacromolecules 16:3357-61.

  • Matthaei, J.F., F. DiMaio, J. Richards, L.D. Pozzo, D. Baker and F. Baneyx. 2015. Designing two-dimensional protein arrays through fusion of multimers and interface mutations. Nano Lett. 15:5235-5239 [cover story]

  • Swift, B. and F. Baneyx. 2015. Microbial uptake, toxicity and fate of biofabricated ZnS:Mn nanocrystals. PLoS One. 10:e0124916.

  • Zhou, W., B. Swift and F. Baneyx. 2015. A minimized designer protein for facile fabrication of ZnS:Mn quantum dots. Chem. Commun. 51:3515-3517 [cover story].