Chemical Engineering
 

David G. Castner
Professor of Chemical Engineering 
and Bioengineering

Contact Information

239A Benson Hall
Box 351750
Seattle, WA 98195-1750		 Phone: 206-543-8094
FAX: 206-543-3778
Email: castner@nb.engr.washington.edu

Education

B.S., Oregon State University, 1975.
Ph.D., University of California (Berkeley), 1979.

For more information, please see the
National ESCA and Surface Analysis Center for Biomedical Problems (NESCA/BIO)
website.

Research Interests

The surface region of a biomaterial is the interface between that material and the biological environment. Thus, the surface structure and composition of a biomaterial mediates the biological reactions that occur when biomaterials are placed into the body. Our research is directed at obtaining detailed information about the surface composition and structure of biomaterials and the interaction of biomolecules with those biomaterials. Recent advances in biochemistry and biomaterials have made it possible to control chemistry on a local scale undreamed of only a few years ago. The dimensions of the lateral chemical variations are diminishing, the complexity of the molecules being introduced at the surface is increasing, and the manipulations of the surface moieties become ever more sophisticated. These advances offer great challenges and opportunities for biomedical surface analysis.
 
Experimental methods we use in our research include x-ray photoelectron spectroscopy (XPS, also known as ESCA), static time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning force microscopy (SFM), near edge x-ray adsorption spectroscopy (NEXAFS), sum frequency generation (SFG) vibrational spectroscopy and surface plasmon resonance (SPR). Current research projects include the characterization of model biomaterial systems (self-assembled monolayers, peptides, ordered proteins, etc.), the development of chemical state imaging methods, and characterization of immobilized proteins. The objective of these research projects is to improve our understanding of the relationship between the surface properties of a biomaterial and the biological reactions occurring on that material by determining, in detail, the composition, structure, spatial distribution, and orientation of surface species.
 
Our research is an integral part of the National ESCA and Surface Analysis Center for Biomedical Problems (NESAC/BIO), an NIH-funded instrumentation center directed by Prof. Castner. NESAC/BIO is dedicated to ensuring that biomedical researchers have the tools to obtain the maximum benefit from the information-rich spectra and images produced by state-of-the-art surface analysis instrumentation, experimental protocols, and data analysis methods. In addition to our basic research projects at NESAC/BIO, we also work with leading biomedical researchers across the country and around the world through NESAC/BIO collaborative research projects.

Selected Recent Publications

  1. “Surface Reactivity Mapping and Correlation of Immobilization Chemistry to Cell Adhesion Sites on Non-Adhesive Hydrogel Thin Films,” H. Takahashi, K. Emoto, M. Dubey, D.G. Castner, D.W. Grainger, Advanced Functional Materials, 18, 2079-2088, 2008.
  2. “Ultra-high Vacuum Surface Analysis Study of Rhodopsin Incorporation into Supported Lipid Bilayers,” R. Michel, V. Subramaniam, S.L. McArthur, B. Bondurant, G.D. de Ambruoso, H.K. Hall Jr., M.F. Brown, E.E. Ross, S.S. Saavedra and D.G. Castner, Langmuir, 24, 4901-4906, 2008.
  3. “Interactions of the Streptococcal C5a Peptidase with Human Fibronectin,” J.R. Hull, G.S. Tamura and D.G. Castner, Acta Biomaterialia, 4, 504-513, 2008.
  4. “XPS, ToF-SIMS, NEXAFS and SPR Characterization of Nitrilotriacetic Acid-Terminated Self-Assembled Monolayers for Controllable Immobilization of Proteins,” F. Cheng, L.J. Gamble and D.G. Castner, Analytical Chemistry, 80, 2564-2573, 2008.
  5. “Nanobiomaterials and Nanoanalysis: Opportunities for Improving the Science to Benefit Biomedical Technologies,” D.W. Grainger and D.G. Castner, Advanced Materials, 20, 867-877, 2008.
  6. “X-ray Photoelectron Spectroscopy, Time-of-flight Secondary Ion Mass Spectrometry and Principal Component Analysis of the Hydrolysis, Regeneration and Reactivity of NHS-containing Organic Thin Films,” F. Cheng, L.J. Gamble, D.W. Grainger and D.G. Castner, Analytical Chemistry, 79, 8781-8788, 2007.
  7. “Structure and Reactivity of Adsorbed Fibronectin Films on Mica,” J.R. Hull, G.S. Tamura and D.G. Castner, Biophysical Journal, 93, 2852-2860, 2007.
  8. “Fluorescence, XPS and ToF-SIMS Surface Chemical State Image Analysis of DNA Microarrays,” C-Y Lee, G.M. Harbers, D.W. Grainger, L.J. Gamble and D.G. Castner, Journal of the American Chemical Society, 129, 9429-9438, 2007.
  9. “Structure and DNA Hybridization Properties of Mixed Nucleic Acid/Maleimide-ethylene glycol Monolayers,” C.-Y. Lee, P.-C. T. Nguyen, D.W. Grainger, L.J. Gamble and D.G. Castner, Analytical Chemistry, 79, 4390-4400, 2007.
  10. “Multivariate Analysis Strategies for Processing ToF-SIMS Images of Biomaterials,” B.J. Tyler, G. Rayal and D.G. Castner, Biomaterials, 28, 2412-2423, 2007.
  11. “Advances in ToF-SIMS Analysis of Protein Films,” R. Michel and D.G. Castner, Surface and Interface Analysis, 38, 1386-1392, 2006.
  12. “Surface Coverage and Structure of Mixed DNA/Alkylthiol Monolayers on Gold: Characterization by XPS, NEXAFS, and Fluorescence Intensity Measurements,” C.-Y. Lee, P. Gong, G.M. Harbers, D.W. Grainger, D.G. Castner and L.J. Gamble, Analytical Chemistry, 78, 3316-3325, 2006.
  13. “Information from Complexity:  Challenges of ToF-SIMS Data Interpretation,” D.J. Graham, M.S. Wagner and D.G. Castner, Applied Surface Science, 252, 6860-6868, 2006.
  14. “The influence of PEG architecture on Protein Adsorption and Conformation,” R. Michel, S. Pasche, M. Textor and D.G. Castner, Langmuir, 21, 12327-12332, 2005.
  15. “Surface Characterization of the Extracellular Matrix Remaining after Cell Detachment from a Thermoresponsive Polymer,” H.E. Canavan, X. Cheng, D.J. Graham, B.D. Ratner and D.G. Castner, Langmuir, 21, 1949-1955, 2005.
  16. “Maximizing Information Obtained from Secondary Ion Mass Spectra of Organic Thin Films Using Multivariate Analysis,” M.S. Wagner, D.J. Graham, B.D. Ratner and D.G. Castner, Surface Science, 570, 78-97, 2004.
  17. "Methods for Generating Protein Molecular Ions in ToF-SIMS," S.L. McArthur, M.C. Vendettuoli, B.D. Ratner and D.G. Castner, Langmuir, 20, 3704-3709, 2004.
  18. "A Streptavidin Linker Layer That Functions After Drying," N. Xia, J. Shumaker-Parry, H. Zareie, C.T. Campbell and D.G. Castner, Langmuir, 20, 3710-3716, 2004.
  19. "Quantitative XPS and ToF-SIMS Characterization of the Components in DNA Microarrays," C.J. May, H.E. Canavan and D.G. Castner, Analytical Chemistry, 76, 1114-1122, 2004.
  20. "Characterization of Poly(L-Lysine)-graft-Poly(Ethylene Glycol) Assembled Monolayers on Niobium Pentoxide Substrates using ToF-SIMS and Multivariate Analysis," M. S. Wagner, S. Pasche, D. G. Castner, and M. Textor, Analytical Chemistry, 76, 1483-1492, 2004.
  21. "Covalent Coupling and Characterization of Supported Lipid Layers," S.L. McArthur, M.W. Halter, V. Vogel and D.G. Castner, Langmuir, 19, 8316-8324, 2003.
  22. "Denoising and Multivariate Analysis of ToF-SIMS Images," B. Wickes, Y. Kim and D.G. Castner, Surface and Interface Analysis, 35, 640-648, 2003.
  23. "Preserving the Structure of Adsorbed Protein Films for Time-of-Flight Secondary Ion Mass Spectrometry Analysis," N. Xia and D.G. Castner, J. Biomed. Mater. Res., 67A, 179-190, 2003.
  24. "ToF-SIMS: Techniques and Applications for the Characterization of Biomaterial Surfaces," A. Belu, D.J. Graham and D.G. Castner, Biomaterials, 24, 3635-3653, 2003.
  25. "Characterization of the Structure of Binary and Ternary Adsorbed Protein Films using Radiolabeling, ESCA and ToF SIMS," M.S. Wagner, T.A. Horbett and D.G. Castner, Langmuir, 19, 1708-1715, 2003.
  26. "Ultrasensitive Probing of the Protein Resistance of PEG Surfaces by Secondary Ion Mass Spectrometry," P. Kingshott, S.L. McArthur, H. Thissen, D.G. Castner and H.J. Griesser, Biomaterials, 23, 4775-4785, 2002.
  27. "ToF-SIMS Analysis of Conformational Changes in Adsorbed Protein Films," N. Xia, C.J. May, S.L. McArthur and D.G. Castner, Langmuir, 18, 4090-4097, 2002.
  28. "Interpretation of ToF-SIMS Spectra of Adsorbed Protein Films by Multivariate Pattern Recognition," M.S. Wagner, B.J. Tyler and D.G. Castner, Anal. Chem., 74, 1824-1835, 2002.
  29. "Surface Structure and Orientation of PTFE Films Determined by Experimental and FEFF8-Calculated NEXAFS Spectra," L.J. Gamble, B. Ravel, D.A. Fischer, and D.G. Castner, Langmuir, 18, 2183-2189, 2002.
  30. "Biomedical Surface Science: Foundations to Frontiers," D.G. Castner and B.D. Ratner, Surface Science, 500, 28-60, 2002.
  31. "Static ToF SIMS and XPS Characterization of Adsorbed Albunim and Fibronectin Films," C.D. Tidwell, D.G. Castner, S.L. Golledge, B.D. Ratner, K. Meyer, B. Hagenoff, and A. Benninghoven, Surface and Interface Analysis, 31, 724-733, 2001.
  32. "Surface Characterization of Mixed Self-Assembled Monolayers Designed for Streptavidin Immobilization," K.E. Nelson, L. Gamble, L.S. Jung, M.S. Boeckl, E. Naeemi, S.L. Golledge, T. Sasaki, D.G. Castner, C.T. Campbell, and P.S. Stayton, Langmuir, 17, 2807-2816, 2001.

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