Shaoyi Jiang

Shaoyi JiangBoeing-Roundhill Professor of Chemical Engineering and Adjunct Professor of Bioengineering

Office: 243 Benson
Phone: 206-616-6509
Fax: 206-685-3451 or 206-543-3778
 
 
 
 
 

Education

 
  • Ph.D., Cornell University, 1993.
  • Postdoctoral Fellow, University of California (Berkeley), 1993-1994.
  • Research Fellow, California Institute of Technology, 1994-1996.
 

Research Interests 

 
  • Biomolecular Interfaces
  • Biomaterials
  • Biosensors
 
 

Molecular Design of Superlow Biofouling Materials: From Molecular Modeling to Product Development

 
Materials and surfaces will face the undesirable accumulation of various organisms when they are in contact with biological media. While uncontrolled biological encapsulation seriously degrades the performance of many implanted devices, biofouling on ships reduces their speed and maneuverability and increases fuel and maintenance costs. Over the last few years, we have demonstrated that zwitterionic and mixed charge materials and surfaces are highly resistant to nonspecific protein adsorption from undiluted blood plasma/serum and to bacterial adhesion/biofilm formation. In addition to their excellent nonfouling properties, zwitterionic carboxybetaine-based materials have abundant functional groups for ligand immobilization, while cationic zwitterionic precursors have self-sterilizing capabilities. Our results show that the strong hydration of zwitterionic materials is responsible for their excellent nonfouling properties. At present, zwitterionic materials have been applied to a number of biomedical and engineering applications such as drug/gene delivery, implanted medical devices, diagnostics, antimicrobial coatings and marine coatings.
 
We take a unique holistic approach to our research by performing both simulations and experiments. Novel materials are designed, synthesized, tested and improved upon all within our group. This collaborative approach allows us to always strive towards understanding nature at all levels. Creative ideas and innovative technologies are always our emphasis.
 
 

Molecular Engineering of Surfaces for Sensing and Detection

 
Surface plasmon resonance (SPR) sensors provide fast, real-time, sensitive, quantitative, specific and label-free detections in small sample volumes. Surface chemistry for the immobilization of biomolecular recognition elements is the key to the success of a sensor. The objective of this work is to develop surface chemistries, allowing high sensitive and specific detection in complex environments. We have developed approaches to convert DNA arrays into protein arrays, which resolve several key issues encountered in current protein array technologies, particularly chip stability and storage. Furthermore, we have developed zwitterionic-based protein arrays to achieve a high signal-to-noise ratio for detection in complex media such as undiluted blood plasma and serum. These new surface chemistries are integrated into our arrayed, imaging, and portable SPR systems. Two focused applications are food safety monitoring in collaboration with researchers from U.S. FDA and U.S. Army and early cancer diagnostics in collaboration with researchers from the Fred Hutchinson Cancer Research Center in Seattle.
 

Molecular Control of Biomolecular Interfaces

 
For protein-surface interactions, we focus on the prediction, control and probing of protein orientation and conformation. We proposed a charge-driven protein orientation principle and demonstrated this principle using IgG1 and IgG2a. Molecular simulations were used to map out protein orientation under various conditions, while time-of-flight secondary-ion mass spectroscopy (ToF-SIMS) and SPR were used to probe protein orientation. This principle was applied successfully to control orientations of several cell-binding proteins, including osteopontin (OPN) and fibronectin (FnIII7-10). In addition, we have also used an alternative approach to control protein orientation via specific protein-protein interactions, e.g., OPN/collagen I. Our recent in vivo studies indicate that the orientation of bound OPN indeed has an effect on the foreign body capsule thickness. For protein-protein interactions, we focus on the identification of protein-protein binding pairs using SPR and locations using AFM, particularly for proteins within the extracellular matrix (ECM). For cell-surface interactions, we focus on the control of angiogenesis. We have studied the differentiation of endothelial cells into capillary-like structures induced by patterned surfaces.
 
 

Selected Recent Publications

 
  • S. Jiang and Z.Q. Cao, Ultralow-Fouling, Functionalizable, and Hydrolyzable Zwitterionic Materials and Their Derivatives for Biological Applications (Invited Perspective Article), Advanced Materials, 22, 920 (2010).
  • Z. Q. Cao, Q. Yu, H. Xue, G. Cheng and S. Jiang, Drug Delivery Nanoparticles From PLGA-zwitterionic Amphiphilic Polymers With Sharp Polarity Contrast Between Two Blocks, Angew. Chem. Int. Ed., 49, 3771 (2010).
  • W. Yang, H. Xue, L. R. Carr, J. Wang, and S. Jiang, Zwitterionic Poly(carboxybetaine) Hydrogels for Glucose Sensors in Complex Media, Biosensors and Bioeletronics, 26, 2454 (2010).
  • A. White and S. Jiang, Local and Bulk Hydration of Zwitterionic Glycine and its Analogues through Molecular Simulations, Journal of Physical Chemistry B, 115, 660 (2011).
  • L. R. Carr, C. Gao, and S. Jiang, Mediating High Levels of Gene Transfer without Cytotoxicity via Hydrolytic Cationic Ester Polymers, Biomaterials, 31, 4186 (2010).
  • S. Chen, Z.Q. Cao, and S. Jiang, Ultra-low Fouling Peptide Surfaces Derived from Natural Amino Acids, Biomaterials, 30, 5892 (2009).
  • W. Yang, H. Xue, W. Li, J. Zhang and S. Jiang, Pursuing “Zero” Protein Adsorption of Poly(carboxybetaine) from Undiluted Blood Serum and Plasma, Langmuir, 25, 11911 (2009).
  • G. Cheng, H. Xue, Z. Zhang, S. Chen, and S. Jiang, Switchable Polymer Surface with Self-sterilizing and Non-fouling/ biocompatible Capabilities, Angewandte Chemie International Edition, 47, 8831 (2008) [highlighted by Science, 322, 651 (2008) and Nature Materials, 7, 834 (2008)].
  • H. Vaisocherová, W. Yang, Z. Zhang, Z. Cao, G. Cheng, Marek Piliarik, Jiří Homola, Shaoyi Jiang, Ultra-low Fouling and Functionalizable Surface Chemistry Based on a Zwitterionic Polymer Enabling Sensitive and Specific Protein Detection in Undiluted Blood Plasma, Analytical Chemistry, 80, 7894 (2008).
  • S. Chen and S. Jiang, A New Avenue to Nonfouling Materials, Advanced Materials, 20, 335 (2008).
  • S. Chen, J. Zheng, L. Li, and S. Jiang, Strong Resistance of Phosphorylcholine Self-Assembled Monolayers to Protein Adsorption: Insights into Nonfouling Properties of Zwitterionic Materials, Journal of the American Chemical Society, 127, 14473 (2005).
  • L. Li, S. Chen, J. Zheng, B. Ratner, and S. Jiang, Protein Adsorption on Oligo(ethylene glycol) Terminated Alkanethiolate Self-Assembled Monolayers: Non-fouling Mechanism on the Molecular Basis, Journal of Physical Chemistry B, 109, 2934 (2005).

Contact Us

Dept. of Chemical Engineering

phone: (206) 543-2250
fax: (206) 543-3778

dand@cheme.washington.edu