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Course Description
Credit 3. For engineers with no prior experience in the biological
sciences. Covers fundamentals and concepts of molecular biology
and directed genetic modification strategies using prokaryotic microorganisms
as examples. Focus on approaches, techniques, and relevance to engineered
systems.
Prerequisite: either CHEM 223, CHEM 237, or CHEM 335; recommended:
either CHEM E 467 or BIOEN 450.
Prerequisites
Either CHEM 223, CHEM 237, or CHEM 335 (organic chemistry); recommended:
either CHEM E 467 (Biochemical Engineering)
or BIOEN 450 (Molecular Biology for Engineers I).
Textbook
Glick and Pasternak, Molecular Biotechnology, Principles and
Applications of Recombinant DNA, ASM Press, 1998.
Course Objectives
- Learn language, techniques and approaches of molecular biology.
- Be able to access current literature in molecular biology.
- Understand the power of molecular biology for engineering applications.
- Design an engineered biological system and write a report about
it.
Topics Covered:
- Introduction to prokaryotic phylogeny and cell biology
- DNA, RNA, protein: structure and information flow
- Methods for analysis of DNA, RNA and protein
- Methods for analysis of DNA, RNA and protein (cont.)
- DNA sequence information manipulation
- Applications Overview; DNA Replication
- Regulation of transcription
- Prokaryotic genetics
- Genetic engineering in prokaryotes: vectors, restriction enzymes
- Genetic engineering: cloning strategies
- PCR and its applications
- Manipulation of gene expression
- Manipulation of gene expression (cont.)
- Directed mutagenesis and protein engineering
- Vaccines
- Bioremediation
- Biomass production
- Genomics
Class Schedule:
Wed/Fri 2:30-4:00 PM (1.5 hrs each)
Contributions of Course to Meeting the Professional Component:
(b) engineering design 100%
Relationship of Course to Program Objectives:
(a) an ability to apply knowledge of mathematics, science, and engineering
(c) an ability to design a system, component, or process to meet
desired needs
(f) an understanding of professional and ethical responsibiltiy
(g) an ability to communicate effectively
(h) the broad education necessary to understand the impact of engineering
solutions in a global and societal context
(j) a knowledge of contemporary issues
Prepared by: Mary E. Lidstrom
Date: April 17, 2001 |
